PCR (Polymerase Chain Reaction)
Analysis of the presence/absence of desired DNA sequences in a sample
through the amplification by the polymerase chain reaction
RFLP (Restriction Fragment Length Polymorphism)
Analysis of the obtained fragments following enzymatic restriction. We will
make a simple or multiple sample digestion, perform an electrophoresis and
detect the resulting fragments.
isoform analysis
Determination of the topoisomer homogeneity in a plasmid sample. We will
perform an electrophoresis on the plasmid preparation, followed by a
densitometry analysis to discriminate and quantify the different topoisomers.
total protein content
Total protein load analysis in a sample through the bicinchonininc acid
assay (BCA).
endotoxin content
Analysis of the endotoxin content in a sample through the chromogenic L
AL (Limulus Amebocyte Lisate) test.
sequencing
Reading of the nucleotide sequence of a DNA fragment through automatic
sequencing.
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